T4 ligase m0202l
WebThis product can be used for the following applications: Cloning of restriction fragments Joining linkers and adapters to blunt-ended DNA WebT4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. Applications ... M0202L -20 : …
T4 ligase m0202l
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Web售价: ¥ 2558.79 原价: ¥ 3159.00: 货号: m0202l : 品牌: neb : 原产地: 分装/原装: 生产商 WebManufacturer: New England Biolabs, Inc. M0202L. The industry standard for performance and quality. T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. Catalog No. 50-811-603. $260.00 / Each of 1.
WebAn IL17 antibody, a preparation method therefor and an application thereof. The IL17 antibody comprises a heavy chain variable region, which comprises CDR1, the sequence of which is shown in any one of SEQ ID NO: 1-24; CDR2, the sequence of which is shown in any one of SEQ ID NO: 25-47; and CDR3, the sequence of which is shown in any one of … WebLigation Protocol with T4 DNA Ligase (M0202) Protocols.io also provides an interactive version of this protocol where you can discover and share optimizations with the …
WebT4 DNA Ligase will ligate these substrates: dsDNA Nicked DNA/RNA Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl … For blunt ends or single base overhangs, incubate at 16°C overnight or room … WebFor cohesive (sticky) ends, use 1 µl of T4 DNA Ligase in a 20 µl reaction for 10 minutes. For blunt ends, use 1 µl of T4 DNA Ligase in a 20 µl reaction for 2 hours or 1 µl high …
WebT4 DNA Ligase from multiple suppliers was tested in reactions containing a fluorescent labeled single stranded, double stranded blunt, 3’overhang or 5’ overhang containing …
WebMar 9, 2024 · We demonstrated that the introduction of chromatin opening elements, UCOE, with the cytomegalovirus (CMV) promoter into the host cell genome can be used as artificial integration sites. 5′2.2A2UCOE-CMV randomly integrated sites improved CRISPR/Cas9-mediated genome editing efficiency including knock-in (KI) efficiency and indel frequency. epidemiology rate definition psychologyWeb10 × Ligase Buffer* T4 DNA Ligase (400 U/μl) C301-01 40,000 U 1 ml 100 μl Components ddH 2O 10 × Ligase Buffer Inserta Vectorb T4 DNA Ligase (400 U/μl) Volume To 10 μl 1 μl 0.3 pmol 0.03 pmol 1 μl Tel: +86 25-83772625 Email: [email protected] Web: www.vazyme.com Loc: Red Maple Hi-tech Industry Park, Nanjing, PRC epidemiology population at riskWebApr 12, 2024 · M0202 - T4 DNA Ligase Revision date 12-Apr-2024 4. First-aid measures Description of first aid measures Inhalation Remove to fresh air. Eye contact Rinse thoroughly with plenty of water for at least 15 minutes, lifting lower and upper eyelids. Consult a physician. Skin contact Wash skin with soap and water. Ingestion Rinse … driver focused interiorWebT4 DNA Ligase, New England Biolabs. Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This … driver fix toolWebJun 18, 2024 · T4 DNA ligase: New England Biolabs: Cat#M0202L: BbsI: New England Biolabs: Cat#R3539S: ... T4 ligase: 1 μL: Total: 10 μL: 8. Transformation. Transfer 10 μL of ligation product into 100 μL of competent Stbl3 strain, incubate the mixture on ice for 1 h, heat-shock at 42°C for 90 s and return it immediately on ice for 3 min. epidemiology of whooping coughWebThe development of efficient methods for the detection of T4 DNA ligase is extremely important for public health. The present work demonstrates the integration of engineerable oxidase nanozyme of LaMnO 3.26 nanomaterials for the colorimetric detection of T4 DNA ligase. Specifically, the LaMnO 3.26 nanomaterials exhibited oxidase-like activity, … epidemiology pima countyWebPlease see the NEB website for more information. driverfix torrent download