Putative endolysin
WebFeb 15, 2024 · The gene (GenBank accession no. OL415943) encoding putative endolysin from bacteriophage PBEC131 was cloned into pET21a+ (Novagen, United States) using … WebPreviously, bioinformatic approaches resulted in a database of 66 SAR endolysins. This manuscript almost doubles the list with 53 additional SAR endolysin candidates. Forty …
Putative endolysin
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WebMar 1, 2024 · that a 36.1-kbp putative phage remnant region exists only on the genome of C. perfringens st13 and is absent from the genomes of the other sequenced C. perfringens strains (Figure S2). A gene designated psa (gene ID; CPE1138), encoding a putative endolysin, was detected at one end of this region; a putative holin gene is also present in … WebJul 21, 2024 · In this study, an open reading frame encoding putative endolysin was cloned from the complete genome data and expressed in the Escherichia coli expression system. …
WebOct 7, 2014 · Resistance rates are increasing among several problematic Gram-negative pathogens, a fact that has encouraged the development of new antimicrobial agents. This paper characterizes a Salmonella phage endolysin (Lys68) and demonstrates its potential antimicrobial effectiveness when combined with organic acids towards Gram-negative … WebThis study investigates 2,628 putative endolysin genes identified in 183,298 bacteriophage genomes. Previously, bioinformatic approaches resulted in a database of 66 SAR-endolysins.
WebApr 12, 2011 · We found that an endolysin gene (psm) in phiSM101, an episomal phage of enterotoxigenic C. perfringens type A strain, SM101 (Myers et al. 2006), encodes a …
WebMay 11, 2024 · The putative endolysin gene was identified from the CPS2 genome and designated as LysCPS2. Pfam and Conserved Domain Database analysis revealed that LysCPS2 is a putative
WebOct 7, 2014 · Resistance rates are increasing among several problematic Gram-negative pathogens, a fact that has encouraged the development of new antimicrobial agents. This … philipp plein coatWebMar 22, 2012 · Computational predictions and analyses of HolSMP. The complete 36,126-bp sequence of phage SMP has 48 open reading frames (ORF). ORF42, designated LySMP, … philipp plein bootsWebAug 6, 2016 · Endolysin from Staphylococcus aureus phage SA97 (LysSA97) was cloned and investigated. LysSA97 specifically lyse the staphylococcal strains and effectively disrupted staphylococcal biofilms. Bioinformatic analysis of LysSA97 revealed a novel putative cell wall binding domain (CBD) as well as two enzymatically active domains … philipp plein customer serviceWebMar 15, 2024 · Bioinformatics analysis using BLAST with LysK endolysin gene sequence as query allowed for the identification of 25 putative endolysin encoding genes. Cg and MatN endolysin encoding genes representing different phylogeny groups (41.53% identity, E = 5.10 –4 ), as determined using MEGA 6.0 molecular evolutionary analysis software, were … trust and vulnerability trauma brene brownWebClostridium perfringens is an often-harmful intestinal bacterium that causes various diseases ranging from food poisoning to life-threatening fulminant disease. Potential treatments include phage-derived endolysins, a promising family of alternative antimicrobial agents. We surveyed the genome of the C. perfringens st13 strain and identified an … philipp plein black fridayWebMar 23, 2024 · To enable functional and structural studies of the putative Enc34 endolysin, the coding sequence of the ORF39 protein with a cleavable N-terminal hexahistidine-tag was cloned and expressed in ... trust and verify reaganWebApr 11, 2024 · Aiming at utilizing a phage endolysin as a therapeutic alternative to antibiotics, we surveyed the genome and bacteriophage sequences of C. perfringens. A phiSM101 muramidase gene (psm) revealed by this study can be assumed to encode an N-acetylmuramidase, since the N-terminal catalytic domain deduced from the gene shows … trust and wealth management