Human monocyte cell line thp-1
WebTo foster research on the ASC adaptor, InvivoGen provides cellular tools that have been generated from the human monocytic THP1 cell line. THP-1 cells are widely used for … WebZinc supplementation trials in the elderly showed that the incidence of infections was decreased by approximately 66% in the zinc group. Zinc supplementation also decreased oxidative stress biomarkers and decreased inflammatory cytokines in the elderly. In our studies in the experimental model of zinc deficiency in humans, we showed that zinc …
Human monocyte cell line thp-1
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WebEndothelial cellular stiffening has been observed not only in inflamed cultured endothelial cells but also in the endothelium of atherosclerotic regions, which is an underlying cause of monocyte adhesion and accumulation. Although recombinant soluble thrombomodulin (rsTM) has been reported to suppress the inflammatory response of endothelial cells, its … WebTHP-1 cells as a model for human monocytes Ann Transl Med. 2016 Nov;4 (21):438. doi: 10.21037/atm.2016.08.53. Authors Herbert Bosshart 1 , Michael Heinzelmann 2 …
Web26 feb. 2024 · THP-1 derived macrophages (THP-1- Mφ) The THP-1 cells were trypsinized with 0.25% trypsin (Gibco) and centrifuged at 1000 rpm for 5 min. The cells were resuspended in RPMI 1640 medium supplemented with 100 ng/mL PMA (Beyotime, Beijing, China) and seeded into six-well plates at 10 6 cells/well. WebBackground. Iron overload is a major health concern for transfusion-dependent patients. Repeated transfusions result in the loading of large amounts of haem-derived iron on macrophages, in turn, inducing cell death.We previously demonstrated that haemin-induced cell death in human monocytic THP-1 cells is consistent with ferroptosis ferroptosis
Web22 uur geleden · THP-1 cells were cultured in RPMI-1640 medium containing 10% fetal bovine serum and 1% penicillin-streptomycin and 2-mercaptoethanol in a 5% CO 2 incubator at 37 °C. After the cells were allowed to proliferate sufficiently, the cells were counted and seeded into 6-well plates, each with 1x10 5 cells in medium. Web1 nov. 2014 · THP-1 is a human leukemia monocytic cell line, which has been extensively used to study monocyte/macrophage functions, mechanisms, signaling pathways, and …
Web1 jul. 1991 · THP-1 cells are a monocyte-like cell line derived from a patient with acute monocytic leukemia and unlike other leukemic cell lines has a normal diploid karyotype. …
Web2 aug. 2012 · The ratio of expression of ASGR1 : ASGR2 in monocytes and the monocyte cell line THP1 was approximately 1 : 100. 3.5. ... Yamaguchi Y. Establishment and characterization of a human acute monocytic leukemia cell line (THP-1) International Journal of Cancer. 1980; 26 (2):171–176. [Google Scholar] nicol. hartmann cie. ag churWebHere, we demonstrate for the first time the expression of MMP-9 by clusterin in human primary monocytes as well as human and murine macrophage cell lines, THP-1, and Raw264.7. MMP-9 expression was accompanied by increased enzymatic activity, as revealed by gelatin zymography. now pe ratioWeb1 jan. 2010 · PDF The effects of a hot water-soluble extract of Grifola frondosa on the viability of multiple immune cell lines were examined in vitro. The extract... Find, read and cite all the research ... nicol hervéWeb6 feb. 2024 · Cultured MLECs also showed functional characteristics of ECs, evidenced by DiI-oxLDL uptake assay and THP-1 monocyte adhesion assay. Finally, we used MLECs from endothelium-specific enhancer of zeste homolog 2 (EZH2) knockout mice to show the general applicability of our protocol. nicol howardWeb5 mei 2014 · Because THP-1 is a spontaneously immortalized human monocytic cell line derived from an acute monocytic leukemia patient, the possibility could not be excluded that the molecular network we identified does not represent the physiological network of non-malignant human monocytes. nico lift off hingesWebHuman Thp 1 Monocyte Macrophage Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - … now performerWebI want to use the PMA solution to stimulate THP-1 (1x106 cells/ml), so I will choose one tube of PMA and dilute it by adding 990uL PBS so that the concentration of PMA reach to 10ug/ml. 25uL... nicol hughes log in